cAMP(Cyclic adenosine monophosphate) ELISA Kit
18500 INR/Kit
Product Details:
- Type ELISA Kit
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cAMP(Cyclic adenosine monophosphate) ELISA Kit Price And Quantity
- 1 Kit
- 18500 INR/Kit
cAMP(Cyclic adenosine monophosphate) ELISA Kit Product Specifications
- ELISA Kit
cAMP(Cyclic adenosine monophosphate) ELISA Kit Trade Information
- 500 Kit Per Day
- 1-7 Days
Product Description
Intended use
This ELISA kit can be applied to the in vitro quantitative determination of cAMP concentrations in serum, plasma and other biological fluids.
Test principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Assay type | Competitive |
| Format | 96T |
| Assay time | 2.5h |
| Reactivity | Universal |
| Detection Method | Colormetric |
| Detection Range | 1.56100 ng/mL |
| Sensitivity | 0.94 ng/mL |
| Sample Volume | 50L |
| Sample Type | Serum, plasma and other biological fluids |
Specificity
This kit recognizes cAMP in samples. No significant cross-reactivity or interference was observed.
Kit components & Storage
The unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 8 wells 12 strips | -20, 6 months |
| Reference Standard | 2 vials | |
| Concentrated Biotinylated Detection Ab (100) | 1 vial, 120 L | |
| Concentrated HRP Conjugate (100) | 1 vial, 120 L | -20(shading light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 4, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer (25) | 1 vial, 30 mL | |
| Substrate Reagent | 1 vial, 10 mL | 4(shading light) |
| Stop Solution | 1 vial, 10 mL | 4 |
| Plate Sealer | 5 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.
Other supplies required
- Microplate reader with 450nm wavelength filter
- High-precision transferpettor, EP tubes and disposable pipette tips
- 37 Incubator
- Deionized or distilled water
- Absorbent paper
- Loading slot for Wash Buffer
Assay Procedure
| 1.Add 50 L of standard or sample to each well. | |
| 2.Immediately add 50 L of Biotinylated Detection Ab to each well. | |
| 3.Incubate for 45 min at 37. Aspirate and wash 3 times. | |
| 4.Add 100 L of HRP Conjugate to each well. Incubate for 30 min at 37. Aspirate and wash 5 times. | |
| 5.Add 90 L of Substrate Reagent. Incubate for 15 min at 37. | |
| 6.Add 50 L of Stop Solution. | |
| 7.Read the OD at 450 nm immediately. Calculation of the results. |
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Contact Details
- Plot No- D17/63, Rohini Sector 3, Near Ram Lila Park, Delhi - 110085, India
- Phone :08045816517
- Send Inquiry
Send Inquiry- Mr Rambhajan Dwivedi (Proprietor)
- Mobile :08045816517
- Customer Care Number---011-27511805
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