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Cat no | size | price | status |
K106-25 | 25 test | 13000/- | In stock |
K106-100 | 100 test | 29,000/- | In stock |
K106 -200 | 200 Test | 48,000/- | 10-12 Days |
Kit Principal- Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
For Research Use Only! Not For Use in Humans
Product Citations-
Anderson, E. J. et. al. Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol, Jan 2011; 300: H118 - H124.
Baker et al., Molecular and Cellular Mechanisms Responsible for Cellular Stress and Low-grade Inflammation Induced by a Super-low Dose of Endotoxin. J. Biol. Chem., Jun 2014; 289: 16262 - 16269.
Carley Glass et. al. MicroRNA-1 transfected embryonic stem cells enhance cardiac myocyte differentiation and inhibit apoptosis by modulating the PTEN/Akt pathway in the infarcted heart. Am J Physiol Heart Circ Physiol, Nov 2011; 301: H2038 - H2049
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