DA(Dopamine) ELISA Kit

18500 INR/Kit

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Type ELISA Kit
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DA(Dopamine) ELISA Kit Price And Quantity

Price 18500 INR/Kit
Minimum Order Quantity 1 Kit

DA(Dopamine) ELISA Kit Product Specifications

Type ELISA Kit

DA(Dopamine) ELISA Kit Trade Information

Supply Ability 500 Kit Per Day
Delivery Time 1-7 Days

Product Description

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of DA concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DA. During the reaction, DA in the sample or standard competes with a fixed amount of DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of DA in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type	Competitive
Format	96T
Assay time	2.5h
Reactivity	Universal
Detection Method	Colormetric
Detection Range	31.252000 pg/mL
Sensitivity	18.75 pg/mL
Sample Volume	50L
Sample Type	Serum, plasma and other biological fluids

Specificity

This kit recognizes DA in samples. No significant cross-reactivity or interference was observed.

Recovery

The recovery of DA spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	95-110	101
EDTA plasma (n=5)	97-111	102
Cell culture media (n=5)	92-103	98

Linearity

Samples were spiked with high concentrations of Human ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	92-106	95-109	91-103
1:2	Average (%)	99	103	98
1:4	Range (%)	91-103	89-105	105
1:4	Average (%)	96	96	105
1:8	Range (%)	91-103	92-104	99-113
1:8	Average (%)	96	99	105
1:16	Range (%)	86-98	91-105	93-108
1:16	Average (%)	92	99	100

Kit components & Storage

The unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells 12 strips	-20, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100)	1 vial, 120 L
Concentrated HRP Conjugate (100)	1 vial, 120 L	-20(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4(shading light)
Stop Solution	1 vial, 10 mL	4
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.