olyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.
Based on immunoelectrophoresis, the antibody reacts with whole molecule chicken IgG. It also reacts with the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Product Specifications
Product: Donkey Anti-Chicken IgY(IgG) (H+L)
Code Number: 703-001-003
Lot Number: 131883
Physical State: Freeze-dried solid
Size: 2.0 ml
Protein Concentration: 44.5 mg/ml
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide
Reconstitution and Storage: Store freeze-dried powder at 2-8C. When ready to use, rehydrate with 2.0 ml dH2O and centrifuge if
not clear. Product is stable for about 6 weeks at 2-8C as an undiluted liquid. Prepare working dilution fresh each day. For
extended storage after rehydration, aliquot and freeze undiluted product at -20C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the
intended use.
Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Antibody Specificity: Based on immunoelectrophoresis, the antibody reacts with whole molecule chicken IgG. It also reacts with
the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The
antibody may cross-react with immunoglobulins from other species.
Country of Origin: USA
Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device.
IgY is the original designation for the IgG-like protein found in both serum and egg yolk.
SR GROUP
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