Human AAA(Anti-Actin Antibody) ELISA Kit

This ELISA kit can be applied to the in vitro quantitative determination of Human AAA concentrations in serum, plasma and other biological fluids.

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AAA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AAA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human AAA. The concentration of Human AAA in samples can be calculated by comparing the OD of the samples to the standard curve.

This kit recognizes Human AAA in samples. No significant cross-reactivity or interference was observed.

Recovery