Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Product Specifications
Product: Normal Alpaca Serum
Code Number: 028-000-001
Lot Number: 130657
Physical State: Freeze-dried solid
Size: 2.0 ml
Protein Concentration: 60.0 mg/ml
Suggested Working Dilution: For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume).
Rehydration according to instruction yields 100% serum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide
Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with 2.0 ml dH2O and centrifuge if
not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For
extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the
intended use.
Process: Normal serum is lipid extracted and dialyzed against the above buffer.
Country of Origin: USA
Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide
SR GROUP
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