Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.
Based on immunoelectrophoresis, the antibody reacts with whole molecule bovine IgG. It also reacts with the light chains of other bovine immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solidExpiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Product Specifications
Product: Rabbit Anti-Bovine IgG (H+L)
Code Number: 301-001-003
Lot Number: 132365
Physical State: Freeze-dried solid
Size: 2.0 ml
Protein Concentration: 41.0 mg/ml
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide
Reconstitution and Storage: Store freeze-dried powder at 2-8C. When ready to use, rehydrate with 2.0 ml dH2O and centrifuge if
not clear. Product is stable for about 6 weeks at 2-8C as an undiluted liquid. Prepare working dilution fresh each day. For
extended storage after rehydration, aliquot and freeze undiluted product at -20C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the
intended use.
Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Antibody Specificity: Based on immunoelectrophoresis, the antibody reacts with whole molecule bovine IgG. It also reacts with the
light chains of other bovine immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The
antibody may cross-react with immunoglobulins from other species.
Country of Origin: USA
Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not
SR GROUP
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