This ELISA kit applies to the in vitro quantitative determination of Rat ACTH concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat ACTH. During the reaction, Rat ACTH in the sample or standard competes with a fixed amount of Rat ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ACTH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.

This kit recognizes Rat ACTH in samples. No significant cross-reactivity or interference between Rat ACTH and analogues was observed.

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.