Rat BK(Bradykinin) ELISA Kit

This ELISA kit applies to the in vitro quantitative determination of Rat BK concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BK. During the reaction, Rat BK in the sample or standard competes with a fixed amount of Rat BK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BK. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat BK in the samples is then determined by comparing the OD of the samples to the standard curve.

This kit recognizes Rat BK in samples. No significant cross-reactivity or interference between Rat BK and analogues was observed.