Rat CDT(Carbohydrate Deficient Transferrin) ELISA Kit

18500 INR/Kit

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Product Details:

Shelf Life 12 Months
Type ELISA Kit
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Rat CDT(Carbohydrate Deficient Transferrin) ELISA Kit Price And Quantity

Price Range 15000.00 - 25000.00 INR/Kit
Minimum Order Quantity 1 Kit
Price 18500 INR/Kit

Rat CDT(Carbohydrate Deficient Transferrin) ELISA Kit Product Specifications

Shelf Life 12 Months
Type ELISA Kit

Rat CDT(Carbohydrate Deficient Transferrin) ELISA Kit Trade Information

Supply Ability 500 Kit Per Day
Delivery Time 1-7 Days

Product Description

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat CDT concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CDT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CDT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CDT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat CDT. You can calculate the concentration of Rat CDT in the samples by comparing the OD of the samples to the standard curve.

Assay type	Sandwich
Format	96T
Assay time	4.5h
Reactivity	Rat
Detection Method	Colormetric
Detection Range	0.6340 nmol/mL
Sensitivity	0.38 nmol/mL
Sample Volume Required Per Well	100 L
Sample Type	Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat CDT in samples. No significant cross-reactivity or interference between Rat CDT and analogues was observed.

Kit components & Storage

An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells 12 strips	-20, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100)	1 vial, 120 L
Concentrated HRP Conjugate (100)	1 vial, 120 L	-20(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4(shading light)
Stop Solution	1 vial, 10 mL	4
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

Microplate reader with 450 nm wavelength filter
High-precision transfer pipette, EP tubes and disposable pipette tips
Incubator capable of maintaining 37
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer
Recovery
The recovery of Rat CDT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range (%) Average Recovery (%)
Serum (n=5) 93-107 99
EDTA plasma (n=5) 94-108 100
Cell culture media (n=5) 85-95 90