5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit
18500 INR/Kit
Product Details:
- Type ELISA Kit
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5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit Price And Quantity
- 1 Kit
- 18500 INR/Kit
5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit Product Specifications
- ELISA Kit
5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit Trade Information
- 500 Kit Per Day
- 1-7 Days
Product Description
Intended use
This ELISA kit can be applied to the in vitro quantitative determination of 5-HIAA concentrations in serum, plasma and other biological fluids.
Test principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 5-HIAA. During the reaction, 5-HIAA in the sample or standard competes with a fixed amount of 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HIAA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of 5-HIAA in the samples is then determined by comparing the OD of the samples to the standard curve.
| Assay type | Competitive |
| Format | 96T |
| Assay time | 2.5h |
| Reactivity | Universal |
| Detection Method | Colormetric |
| Detection Range | 1.56100 ng/mL |
| Sensitivity | 0.94 ng/mL |
| Sample Volume | 50L |
| Sample Type | Serum, plasma and other biological fluids |
Specificity
This kit recognizes 5-HIAA in samples. No significant cross-reactivity or interference was observed.
Kit components & Storage
The unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 8 wells 12 strips | -20, 6 months |
| Reference Standard | 2 vials | |
| Concentrated Biotinylated Detection Ab (100) | 1 vial, 120 L | |
| Concentrated HRP Conjugate (100) | 1 vial, 120 L | -20(shading light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 4, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer (25) | 1 vial, 30 mL | |
| Substrate Reagent | 1 vial, 10 mL | 4(shading light) |
| Stop Solution | 1 vial, 10 mL | 4 |
| Plate Sealer | 5 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.
Other supplies required
- Microplate reader with 450nm wavelength filter
- High-precision transferpettor, EP tubes and disposable pipette tips
- 37 Incubator
- Deionized or distilled water
- Absorbent paper
- Loading slot for Wash Buffer
Assay Procedure
| 1.Add 50 L of standard or sample to each well. | |
| 2.Immediately add 50 L of Biotinylated Detection Ab to each well. | |
| 3.Incubate for 45 min at 37. Aspirate and wash 3 times. | |
| 4.Add 100 L of HRP Conjugate to each well. Incubate for 30 min at 37. Aspirate and wash 5 times. | |
| 5.Add 90 L of Substrate Reagent. Incubate for 15 min at 37. | |
| 6.Add 50 L of Stop Solution. | |
| 7.Read the OD at 450 nm immediately. Calculation of the results. |
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Contact Details
- Plot No- D17/63, Rohini Sector 3, Near Ram Lila Park, Delhi - 110085, India
- Phone :08045816517
- Send Inquiry
Send Inquiry- Mr Rambhajan Dwivedi (Proprietor)
- Mobile :08045816517
- Customer Care Number---011-27511805
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